“Fast” HPLC better than UHPLC
Posted in HPLC on Jul 31, 2007
Research scientists from Schering-Plough Research Institute developed a rapid high-performance liquid chromatography (HPLC) technique that allows for efficient separation and analysis of pharmaceutical compounds.
The method uses a short column packed with 2.7μm “fused-core” silica particles that are made by fusing a 0.5μm layer of porous silica onto a solid silica particle[1]. These unique particles enable very rapid chromatographic separation at a relatively low backpressure.
Ultra-high-pressure liquid chromatography (UHPLC) is another chromatographic technique that allows the system to handle the high backpressure resulting from the stationary phase with sub-2μm particles. UHPLC offers advantages in chromatographic resolution, speed, and sensitivity over conventional HPLC systems.
Newly developed fast HPLC technology is comparable with UHPLC in terms of chromatographic performance but requires neither expensive ultra-high-pressure instrumentation nor new laboratory protocols.
1. “Fused-Core Silica Column High-Performance Liquid Chromatography/Tandem Mass Spectrometric Determination of Rimonabant in Mouse Plasma”, Yunsheng Hsieh et la., Analytical Chemistry, DOI: 10.1021/ac070343g
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I checked both column “Fast” HPLC and UHPLC for my food testing application. Both of them, it has pros and cons.
You mean to say that we dont need ultra high pressure Pump ( 18000psi) ? If you are right, how can you feed eluent into 2.7um paricle packed Column . Need to be seen to beleive it
JustChromatography: you should not accept comments or replies fomr people who don’t identify themselves.
what is stationaryphase and mobile phase and their uses
I compared both methods they are almost the same. I used the narrow-bore and short column, they come out with the fantastic selectivity, speed, and sensitivity.
This post is not Apt with respect to recent innovations in HPLC.
The sub 2u Particles (UPLC) proved to be miles ahead of Fast LC in terms of quality of separation.
I am using Waters UPLC as well as many other Fast LCs and concluded this based on my experience.
I want to know which one is more efficient HPLC or UHPLC…
“whar” no “what”!!!!!excuse me.
labrat: you missed “but requires neither expensive ultra-high-pressure instrumentation nor new laboratory protocols.”
Therefore, it doesnt require buying a new UHPLC system.
I’d consider that to be ‘better’
Your Headline is misleading…
performance is comparable not better.
“This fast HPLC technology is comparable with ultrahigh-pressure liquid chromatography (UHPLC) in terms of chromatographic performance…”
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