A breakthrough patent awarded to a New Jersey Institute of Technology (NJIT) researcher will enable manufacturers to create a device to detect miniscule amounts of airborne pollutants. Using computer chip technology, Somenath Mitra, PhD, professor and chair of NJIT’s Department of Chemistry and Environmental Sciences, has developed and patented what could eventually become a simple […]

Scientists of the University of Twente (The Netherlands) succeed in speeding up the fluid flow in a micro channel using tiny and rapidly imploding gas bubbles that also provide a new way of mixing fluids within a lab-on-a-chip, without the need of complicated external components. The scientists led by Dr. Claus-Dieter Ohl of the MESA+ […]

Shortly after I posted “Proteomics made faster” I saw another research article published on the topic. Now, Detlev Belder and colleagues at the University of Regensburg, have developed a glass microchip that directly couples electrophoretic separation techniques with the electrospray process. As per “Chemistry World“: Chemists in Germany have created a glass microchip that combines […]

The large-scale studies of structure and function of proteins (proteomics) are performed using a variety of analytical techniques from two-dimensional gel electrophoresis, immunoaffinity chromatography to tandem and MALDI-TOF mass spectrometry. The words “large-scale” in proteomics definition refers to the fact that the number of proteins that need to be evaluated is enormous. For example, the […]

Almost two weeks ago there was a question regarding Nano HPLC technology that I attempted to answer and in that post I mentioned Agilent as one of the pioneers in the Nano LC field. Agilent’s “nano” workhorse is a 1200 Series HPLC-Chip/MS system, which uses microfluidics chip-based technology for nanospray LC/MS. Based on the HPLC-Chip […]

The HPLC method used a C8 column and the potassium chloride in aqueous acetic acid as a mobile phase with a linear gradient. The retention time was a bit over 20 min and the identity of the peak was confirmed as furosine by MS (mass spectrometry).

Nano HPLC is a relatively new development in chromatography world driven by recent advancements in proteomics that would require decreasing of inner diameter (ID) of liquid chromatography (LC) column to allow for a smaller sample amount and to increase sensitivity.