High-Performance Liquid Chromatography
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High-performance liquid chromatography (HPLC) also know as high-pressure liquid chromatography is an instrumental system based on chromatography that is widely used in forensic science. The “HP” portion of the acronym is sometimes assigned to the words high pressure (versus high performance), but it refers to the same analytical system. HPLC is used in drug analysis, toxicology, explosives analysis, ink analysis, fibers, and plastics to name a few forensic applications.
Like all chromatography, HPLC is based on selective partitioning of the molecules of interest between two different phases. Here, the mobile phase is a solvent or solvent mix that flows under high pressure over beads coated with the solid stationary phase. While traveling through the column, molecules in the sample partition selectively between the mobile phase and the stationary phase. Those that interact more with the stationary phase will lag behind those molecules that partition preferentially with the mobile phase. As a result, the sample introduced at the front of the column will emerge in separate bands (called peaks), with the bands emerging first being the components that interacted least with the stationary phase and as a result moved quicker through the column. The components that emerge last will be the ones that interacted most with the stationary phase and thus moved the slowest through the column. A detector is placed at the end of the column to identify the components that elute. Occasionally, the eluting solvent is collected at specific times correlating to specific components. This provides a pure or nearly pure sample of the component of interest. This technique is sometimes referred to as preparative chromatography.
Many different types of detectors are available for HPLC. The simplest and least expensive is the refractive index detector (RI). Although this detector is a universal detector, meaning it will respond to any compound that elutes, it does not respond well to very low concentrations and as a result is not widely used. On the other hand, detectors based on the absorption of light in the ultraviolet and visible ranges (UV/VIS detectors and UV/VIS spectrophotometers) are the most commonly used, responding to a wide variety of compounds of forensic interest with good to excellent sensitivity. The photodiode array detector (PDA) is especially useful since it can produce not only a peak-based output (a chromatogram) but also a UV/VIS scan of every component. In many ways, the ideal detector for HPLC is a mass spectrometer (MS), which provides both quantitative information and in most cases a definitive identification of each component (qualitative information). However, HPLC-MS systems are relatively complex and expensive and are not readily available in all labs. Other detectors that are sometimes used include fluorescence detectors (which are very sensitive) and electrochemical detectors.
Unlike in gas chromatography (GC) in which the mobile phase is an inert gas, the mobile phase in HPLC can be one of many different solvents or combinations of solvents. This imparts to HPLC a greater flexibility and range of application than has GC. Because the sample does not have to be converted to the gas phase, compounds such as explosives that break down at high temperatures are much more amenable to HPLC than GC. For HPLC, all that is required is that the sample be soluble in the solvents selected for the analysis. In addition, there are several types of HPLC defined by the type of mobile phase and stationary phase that is used. For forensic applications, one of the most commonly used types of HPLC is referred to as “reversed phase.” In this type of HPLC, the mobile phase is a solvent or mix of solvents that are “polar,” meaning that different parts of the individual solvent molecules carry a partial positive or negative charge. Water, methanol (methyl alcohol), ethanol (ethyl alcohol), and acetone are examples of polar solvents. The stationary phase in reverse phase HPLC is a nonpolar material such as a long chain hydrocarbon molecule. In this type of HPLC, components in the sample will partition and separate based on their degree of interaction with the stationary phase relative to the mobile phase. In other words, the separation is based primarily on the relative polarity of the sample molecules. Reverse phase HPLC is used in drug analysis (LSD for example), analysis of cutting agents such as sugars, explosives, and gunshot residue (GSR), and forensic toxicology.
Normal phase HPLC uses a polar stationary phase and a nonpolar mobile phase, but this is not widely used in forensic applications. Size exclusion chromatography (SEC) is more common and separates compounds based on relative sizes. The stationary phase in SEC is composed of a gel with different sizes of microscopic pores through it. The larger the molecule, the longer it takes for it to navigate through the pores and reach the detector. SEC is useful for the analysis of large molecules that come in a range of sizes such as plastic polymers, proteins, and nitrocellulose, a component of GSR. Chiral chromatography, a relatively recent development, is making inroads into forensic science since it is capable of separating enantiomers, molecules that are mirror images of each other. This capability is particularly valuable in forensic toxicology and drug analysis. Finally, ion exchange chromatography is available for detection species such as nitrate (NO,3-) and other ions.
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Oh my goodness! Awesome article dude! Thank you, However I am encountering difficulties with your RSS. I don’t know the reason why I can’t subscribe to it. Is there anybody having similar RSS issues? Anyone who knows the answer will you kindly respond? Thanx!!
{Tôi hạnh phúc hơn cả vui để tìm thấy trang web này. Tôi muốn cảm ơn bạn {vì đã|dành thời gian cho|chỉ vì điều này|vì điều này|cho bài đọc tuyệt vời này!! Tôi chắc chắn yêu thích từng của nó và tôi cũng đã lưu làm mục ưa thích để xem điều mới trên trang web của bạn.|Tôi có thể chỉ nói rằng thật nhẹ nhõm để khám phá một người mà thực sự hiểu họ là gì đang nói về trên mạng. Bạn chắc chắn nhận ra cách đưa một rắc rối ra ánh sáng và làm cho nó trở nên quan trọng. Nhiều người hơn nữa cần phải đọc điều này và hiểu khía cạnh này của. Tôi không thể tin bạn không nổi tiếng hơn vì bạn chắc chắn có món quà.|Xuất sắc bài viết. Tôi chắc chắn yêu thích trang web này. Cảm ơn!|Thật khó tìm những người có kinh nghiệm về điều này, nhưng bạn nghe có vẻ bạn biết mình đang nói gì! Cảm ơn|Bạn nên tham gia một cuộc thi dành cho một blog trực tuyến tuyệt vời nhất. Tôi sẽ Rất khuyến nghị blog này!|Một hấp dẫn chắc chắn đáng giá bình luận. Tôi nghĩ rằng bạn nên viết thêm về chủ đề này, nó có thể không là một điều cấm kỵ chủ đề nhưng nói chung mọi người không nói về vấn đề như vậy. Đến phần tiếp theo! Chúc mừng.|Xin chào! Tôi chỉ muốn cho bạn một rất to cho thông tin tuyệt vời bạn có ở đây trên bài đăng này. Tôi đang trở lại trang web của bạn để biết thêm thông tin sớm nhất.|Sau khi tôi ban đầu để lại bình luận tôi có vẻ như đã nhấp vào hộp kiểm -Thông báo cho tôi khi có bình luận mới- và bây giờ mỗi lần được thêm vào tôi nhận được bốn email có cùng nội dung. Có lẽ có một cách bạn có thể xóa tôi khỏi dịch vụ đó không? Cảm ơn bạn.|Lần sau nữa Tôi đọc một blog, Tôi hy vọng rằng nó không làm tôi thất vọng nhiều như bài này. Ý tôi là, Vâng, đó là sự lựa chọn của tôi để đọc hết, tuy nhiên tôi thực sự nghĩ bạn sẽ có điều gì đó hữu ích để nói về. Tất cả những gì tôi nghe được là một loạt rên rỉ về điều gì đó mà bạn có thể sửa nếu bạn không quá bận tìm kiếm sự chú ý.|Đúng với bài viết này, tôi thực sự nghĩ trang web này cần nhiều hơn nữa sự chú ý.
{Tôi đã hạnh phúc hơn cả vui khám phá trang web tuyệt vời này. Tôi muốn cảm ơn bạn {vì đã|dành thời gian cho|chỉ vì điều này|vì điều này|cho bài đọc tuyệt vời này!! Tôi chắc chắn thích thú từng của nó và tôi đã đã lưu vào mục ưa thích để xem điều mới trong trang web của bạn.|Tôi có thể chỉ nói rằng thật nhẹ nhõm để khám phá một người mà thực sự biết họ là gì thảo luận trên internet. Bạn chắc chắn hiểu cách đưa một rắc rối ra ánh sáng và làm cho nó trở nên quan trọng. Nhiều người hơn nữa phải xem điều này và hiểu khía cạnh này câu chuyện của bạn. Tôi đã ngạc nhiên bạn không nổi tiếng hơn vì bạn chắc chắn có món quà.|Rất hay bài đăng. Tôi chắc chắn yêu thích trang web này. Tiếp tục làm tốt!|Thật gần như không thể tìm thấy những người có kinh nghiệm trong chủ đề cụ thể này, tuy nhiên, bạn có vẻ bạn biết mình đang nói gì! Cảm ơn|Bạn cần tham gia một cuộc thi dành cho một trang web trên mạng tuyệt vời nhất. Tôi sẽ Rất khuyến nghị trang web này!|Một cuộc thảo luận thú vị đáng giá bình luận. Không còn nghi ngờ gì nữa rằng bạn nên xuất bản thêm về chủ đề này, nó có thể không là một điều cấm kỵ vấn đề nhưng điển hình mọi người không nói về vấn đề như vậy. Đến phần tiếp theo! Chúc mọi điều tốt đẹp nhất!|Chào bạn! Tôi chỉ muốn cho bạn một rất to cho thông tin tuyệt vời bạn có ngay tại đây trên bài đăng này. Tôi đang trở lại trang web của bạn để biết thêm thông tin sớm nhất.|Sau khi tôi ban đầu bình luận tôi có vẻ như đã nhấp vào hộp kiểm -Thông báo cho tôi khi có bình luận mới- và bây giờ mỗi lần được thêm vào tôi nhận được 4 email có cùng nội dung. Có lẽ có một phương pháp dễ dàng bạn có thể xóa tôi khỏi dịch vụ đó không? Cảm kích.|Lần sau Tôi đọc một blog, Hy vọng rằng nó không thất bại nhiều như bài này. Ý tôi là, Tôi biết điều đó là sự lựa chọn của tôi để đọc hết, dù sao thì tôi thực sự tin bạn sẽ có điều gì đó hữu ích để nói về. Tất cả những gì tôi nghe được là một loạt rên rỉ về điều gì đó mà bạn có thể sửa nếu bạn không quá bận tìm kiếm sự chú ý.|Đúng với bài viết này, tôi thực sự tin rằng trang web tuyệt vời này cần nhiều hơn nữa sự chú ý.
https://www.tumblr.com/bongdalu1388
{Tôi rất hài lòng khám phá trang web này. Tôi muốn cảm ơn bạn {vì đã|dành thời gian cho|chỉ vì điều này|vì điều này|cho bài đọc tuyệt vời này!! Tôi chắc chắn đánh giá cao từng một chút nó và tôi đã đánh dấu để xem những thứ mới trên trang web của bạn.|Tôi có thể chỉ nói rằng thật nhẹ nhõm để tìm thấy một cá nhân mà thực sự hiểu họ là gì đang nói về trên mạng. Bạn chắc chắn nhận ra cách đưa một rắc rối ra ánh sáng và làm cho nó trở nên quan trọng. Nhiều người hơn nữa phải đọc điều này và hiểu khía cạnh này của. Tôi đã ngạc nhiên rằng bạn không nổi tiếng hơn vì bạn chắc chắn nhất có món quà.|Xuất sắc bài viết. Tôi hoàn toàn yêu thích trang web này. Tiếp tục với nó!|Thật khó đến những người hiểu biết cho điều này, nhưng bạn nghe có vẻ bạn biết mình đang nói gì! Cảm ơn|Bạn cần là một phần của một cuộc thi dành cho một blog trên mạng tốt nhất. Tôi chắc chắn sẽ khuyến nghị blog này!|Một cuộc thảo luận thú vị chắc chắn đáng giá bình luận. Không còn nghi ngờ gì nữa rằng bạn cần viết thêm về chủ đề này, nó có thể không là một điều cấm kỵ vấn đề nhưng thường xuyên mọi người không nói về vấn đề những điều này. Đến phần tiếp theo! Chúc mừng!|Xin chào! Tôi chỉ muốn cho bạn một rất cho thông tin xuất sắc bạn có ngay tại đây trên bài đăng này. Tôi sẽ là trở lại trang web của bạn để biết thêm thông tin sớm nhất.|Sau khi tôi ban đầu để lại bình luận tôi có vẻ như đã nhấp vào hộp kiểm -Thông báo cho tôi khi có bình luận mới- và bây giờ bất cứ khi nào có bình luận được thêm vào tôi nhận được bốn email có cùng nội dung. Có lẽ có một phương tiện bạn có thể xóa tôi khỏi dịch vụ đó không? Cảm ơn.|Lần sau Tôi đọc một blog, Tôi hy vọng rằng nó không thất bại nhiều như bài này. Ý tôi là, Tôi biết điều đó là sự lựa chọn của tôi để đọc hết, tuy nhiên tôi thực sự tin có lẽ có điều gì đó hữu ích để nói về. Tất cả những gì tôi nghe được là một loạt tiếng rên rỉ về điều gì đó mà bạn có thể sửa nếu bạn không quá bận tìm kiếm sự chú ý.|Đúng với bài viết này, tôi hoàn toàn nghĩ trang web này cần nhiều hơn nữa sự chú ý.
{Tôi khá hài lòng để tìm thấy trang web tuyệt vời này. Tôi muốn cảm ơn bạn {vì đã|dành thời gian cho|chỉ vì điều này|vì điều này|cho bài đọc tuyệt vời này!! Tôi chắc chắn thích từng một phần nó và tôi cũng đã đánh dấu để xem thông tin mới trong trang web của bạn.|Tôi có thể chỉ nói rằng thật thoải mái để khám phá một người mà thực sự biết họ là gì thảo luận trên mạng. Bạn thực sự biết cách đưa một vấn đề ra ánh sáng và làm cho nó trở nên quan trọng. Nhiều người hơn cần kiểm tra điều này và hiểu khía cạnh này câu chuyện của bạn. Thật ngạc nhiên bạn không nổi tiếng hơn vì bạn chắc chắn nhất có món quà.|Rất tốt bài viết. Tôi chắc chắn đánh giá cao trang web này. Tiếp tục viết!|Thật khó tìm những người có học thức trong chủ đề cụ thể này, nhưng bạn nghe có vẻ bạn biết mình đang nói gì! Cảm ơn|Bạn nên tham gia một cuộc thi dành cho một blog trên web hữu ích nhất. Tôi sẽ khuyến nghị trang web này!|Một hấp dẫn chắc chắn đáng giá bình luận. Tôi tin rằng bạn nên viết thêm về chủ đề này, nó có thể không là một điều cấm kỵ vấn đề nhưng nói chung mọi người không thảo luận vấn đề như vậy. Đến phần tiếp theo! Chúc mọi điều tốt đẹp nhất!|Xin chào! Tôi chỉ muốn cho bạn một rất cho thông tin xuất sắc bạn có ở đây trên bài đăng này. Tôi sẽ là quay lại trang web của bạn để biết thêm thông tin sớm nhất.|Khi tôi ban đầu để lại bình luận tôi có vẻ như đã nhấp vào hộp kiểm -Thông báo cho tôi khi có bình luận mới- và bây giờ mỗi lần được thêm vào tôi nhận được bốn email cùng chính xác một bình luận. Có lẽ có một phương tiện bạn có thể xóa tôi khỏi dịch vụ đó không? Cảm kích.|Lần sau Tôi đọc một blog, Hy vọng rằng nó không làm tôi thất vọng nhiều như bài này. Ý tôi là, Tôi biết điều đó là sự lựa chọn của tôi để đọc hết, nhưng tôi thực sự nghĩ bạn sẽ có điều gì đó thú vị để nói. Tất cả những gì tôi nghe được là một loạt rên rỉ về điều gì đó mà bạn có thể sửa nếu bạn không quá bận tìm kiếm sự chú ý.|Đúng với bài viết này, tôi thực sự tin rằng trang web tuyệt vời này cần nhiều hơn nữa sự chú ý.
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[…] from Schering-Plough Research Institute developed a rapid high-performance liquid chromatography (HPLC) technique that allows for efficient separation and analysis of pharmaceutical […]
[…] was released. The new edition avoids well-established theoretical and practical aspects of HPLC that were extensively covered in the first edition published in 1998. However, the editors greatly […]
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[…] High-Performance Liquid Chromatography – High-performance liquid chromatography (HPLC) also know as high-pressure … The stationary phase in reverse phase HPLC is a … Normal phase HPLC uses a polar stationary phase and … […]
[…] was released. The new edition avoids well-established theoretical and practical aspects of HPLC that were extensively covered in the first edition published in 1998. However, the editors greatly […]
[…] Panel 3: Who’s Making Progress Against Counterfeit Drugs? After a great lunch and a great talk by Bejon Misra, Healthy You Foundation (India). We all proceeded back to the conference room and heard from more speakers on this topic. Dr. Michael Green, Centers for Disease Control and Prevention, talked about PH levels in counterfeits and how you can tell they are fake because of the levels in them. There are simple field tests that can be done and there are tests where you collect the drug sample and sent it to a lab equipped with a HPLC. […]
[…] from Schering-Plough Research Institute developed a rapid high-performance liquid chromatography (HPLC) technique that allows for efficient separation and analysis of pharmaceutical […]
Dear Sir/Madam,
Good afternoon!!
Hope everything is going well with you.
We introduce ourselves as a top ranking Trading Company in Bangladesh, engaged in sales & marketing of Pharmaceuticals Machinery last 17 years.
For your information, with our qualified personnel, we are enjoying excellent rapport with our all Pharmaceutical customers.
We are very much interested to sell your product in Bangladesh Market.
Right now we have got a firm inquiry for High Performance Liquid Chromatography (HPLC) from our Valued Customer Ibn Sina Pharma.
The Specification as follows:
 High Performance Liquid Chromatography (HPLC), Quantity: 1 No [Pls see the URS in attachment]
So, please send quotation on C&F C10% Chittagong by sea basis with details technical specification & scan copy of catalogues at the earliest.
For your kind information, we have a very good relation with this customer.
We are eagerly waiting to receive your quotation.
Hope to get your prompt feedback pls!!!
Best Regards,
Anwarul Islam
CEO
==============================================
ASN Corporation
15/16 Tajmahal Road (3rd Floor)│Block # C│Mohammadpur│Dhaka-1207│Bangladesh.
Tel.: +88-02-9129956│Fax: +88-02-9126690│Mobile: +88-01711 547760
E-mail: asnint1@citech.net│asnint@gmail.com
[…] “Soy Milk, Good or Bad? HPLC vs CZE – Part 2” – the post about a study that compares HPLC and CZE methods for QC of soy […]
[…] either from fused silica or PEEK materials currently found in your everyday Gas Chromatography or HPLC, […]