Sample preparation is a crucial step in any analytical method, especially in chromatography where samples have to be homogenous, free of interferences and safe for the column. Today, one of the most widely used sample preparation methods is solid-phase extraction (SPE) that in the mid 1990s largely replaced the conventional but time consuming liquid-liquid extraction […]
Folks from India extracted 99.6% pure eicosapentaenoic acid (EPA) using silver ion (argentation) column chromatography from hydrolyzed sardine oil. The individual fatty acids were determined as fatty acid methyl esters (FAME) by gas-liquid chromatography and gas chromatography-mass spectroscopy as FAME and N-acyl pyrrolidides.