Today there was another question about chromatography on “Yahoo! Answers” from naisy428:
What chemicals can be detected using a Gas Chromatography?
Here is my very short, simplified answer. There are actually two types of Gas Chromatography – Gas-Solid and Gas-Liquid depending on the kind of column is used:
- GSC (Gas-Solid Chromatography) allows to separate and hence detect low-molecular-mass gases such as air component (nitrogen, oxygen, CO2), hydrogen sulfide, carbon monoxide, and nitrogen oxides.
- GLC (Gas-Liquid Chromatography) – this type is very widespread in all fields of science and that’s why when people say “GC” they usually refer to gas-liquid chromatography. This type of GC is mostly used to determine organic compounds such as hydrocarbons, polynuclear aromatics, fatty acids, alcohols, ethers, essential oils or any other volatile compound. What GC can detect greatly depends on the column and detector used. Generally speaking, GC is not suitable for thermally unstable samples and inorganic compounds. For example, you cannot analyze a water sample on GC to test for heavy metals or halides.
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I see you answered a question from Yahoo Answers. Wouldn’t be a good idea for you to accept relevant questions on a separate section of this site directly? Something like “Chemist answers”?
This would be my first question: “Can Chromatography help in any way FISH ( Fluorescence microscopy)? If yes, how?”
Priyadarshan
Savyasachi,
The majority of GC tests on blood are done by forensic labs to determine the amount of toxic substances such as drugs and alcohol. How GC is used in forensics will probably require a separate article that I might do later. You can also test blood for fatty acids (lipids) on GC with Mass Spectrometer detector, a so-called GC/MS.
Technically speaking, human plasma is not different from any another sample so you can test blood for any organic matter on GC.
Please tell me what is revealed when you use chromotograpy on blood.
I see. Try to get your hands on a lot of instrumentations and hopefully the place has some really nice new machines :)
After graduation I did the same thing. I liked so many things in Chemistry that I was not ready to commit myself to a very narrow field while in grad school. So I decided to work in the industry for a year, but ended up working for almost 3 years before I was able to go back to school. It is very easy to get sucked in, you start making money and then it is very hard to go back to be a poor grad student :) doing TA or something.
man, my poor blog got hammered today, or as they say “slashdoted”. I’ve had almost 7000 people come so far. I am glad it survived the people surge – 10 requests per sec is no joke.
I haven’t decided, but I have already signed a contract, so I’ll be doing analytical work for a year…after that, I don’t know.
Mitch,
Chemicalforms.com is an extremely good place for questions like that. I hope naisy428 will read your comment and visits the forum with his/her analytical chemistry questions.
Ψ*Ψ,
GC/MS is a great tool, it can ID anything organic.
But I thought you have not decided where you wanna go after graduation.
If you like Analytical type questions. :)
http://www.chemicalforums.com/index.php?board=8.0
Mitch
GC/MS will likely be my life after graduation. Hopefully they will stick me with the PAH analysis.