Captured by the surface of adsorbent’s solid body, molecules can transfer back into solution – eluent, and then again get adsorbed and so on, changing its state infinite number of times.

Another great LC system by Agilent provides up to 20 times faster analysis and 60 % higher resolution than conventional HPLC without sacrificing resolution, precision or sensitivity and while keeping system pressure at a minimum.

Professor Tsvet filled a glass tube with finely ground powder of pure chalk, damped it with benzene, and then poured over a little bit of chlorophyll solution extracted from a fresh leaf. The very top layer of the powder was, of course, immediately colored green, and then Tsvet proceeded to slowly, drop by drop, add benzene, into the tube filled with chalk.

Mikhail Tsvet discovered and applied essentially a new method of analysis – chromatography.